Journal: Frontiers in Pharmacology

Article Title: Construction and Analysis of circRNA-miRNA-mRNA Molecular Regulatory Networks During Herba Gelsemium elegans Intoxication

doi: 10.3389/fphar.2019.01217

Figure Lengend Snippet: Three significantly up/downregulated circRNAs.

Article Snippet: The labeled mRNAs and circRNAs were separately hybridized onto the Whole Mouse Genome Oligo Microarray (4×44K, Agilent Technologies, Santa Clara, CA) and the Arraystar Mouse circRNA Array V2 (8×15K, Arraystar Inc., Rockville, MD).

Techniques:

Journal: Frontiers in Pharmacology

Article Title: Construction and Analysis of circRNA-miRNA-mRNA Molecular Regulatory Networks During Herba Gelsemium elegans Intoxication

doi: 10.3389/fphar.2019.01217

Figure Lengend Snippet: Cluster analysis of differentially expressed (A) circRNAs and (B) mRNAs. Red represents high expression levels; green represents low expression levels; each row represents a circRNA/RNA; each column represents the expression profile of a tissue sample.

Article Snippet: The labeled mRNAs and circRNAs were separately hybridized onto the Whole Mouse Genome Oligo Microarray (4×44K, Agilent Technologies, Santa Clara, CA) and the Arraystar Mouse circRNA Array V2 (8×15K, Arraystar Inc., Rockville, MD).

Techniques: Expressing

Journal: Frontiers in Pharmacology

Article Title: Construction and Analysis of circRNA-miRNA-mRNA Molecular Regulatory Networks During Herba Gelsemium elegans Intoxication

doi: 10.3389/fphar.2019.01217

Figure Lengend Snippet: CircRNAs and their targeted miRNAs.

Article Snippet: The labeled mRNAs and circRNAs were separately hybridized onto the Whole Mouse Genome Oligo Microarray (4×44K, Agilent Technologies, Santa Clara, CA) and the Arraystar Mouse circRNA Array V2 (8×15K, Arraystar Inc., Rockville, MD).

Techniques:

Journal: Frontiers in Pharmacology

Article Title: Construction and Analysis of circRNA-miRNA-mRNA Molecular Regulatory Networks During Herba Gelsemium elegans Intoxication

doi: 10.3389/fphar.2019.01217

Figure Lengend Snippet: Binding site of mmu_circRNA_013703 and mmu-miR-361-3p. The upper part is the sequence at the junction of circRNA and the lower part is the sequence of miRNA.

Article Snippet: The labeled mRNAs and circRNAs were separately hybridized onto the Whole Mouse Genome Oligo Microarray (4×44K, Agilent Technologies, Santa Clara, CA) and the Arraystar Mouse circRNA Array V2 (8×15K, Arraystar Inc., Rockville, MD).

Techniques: Binding Assay, Sequencing

Journal: Frontiers in Pharmacology

Article Title: Construction and Analysis of circRNA-miRNA-mRNA Molecular Regulatory Networks During Herba Gelsemium elegans Intoxication

doi: 10.3389/fphar.2019.01217

Figure Lengend Snippet: The contrast between qRT-PCR and microarray analysis (n = 3). The vertical axis shows the percentage of each circRNA detected by RT-qPCR and microarray respectively.

Article Snippet: The labeled mRNAs and circRNAs were separately hybridized onto the Whole Mouse Genome Oligo Microarray (4×44K, Agilent Technologies, Santa Clara, CA) and the Arraystar Mouse circRNA Array V2 (8×15K, Arraystar Inc., Rockville, MD).

Techniques: Quantitative RT-PCR, Microarray

Journal: Frontiers in Pharmacology

Article Title: Construction and Analysis of circRNA-miRNA-mRNA Molecular Regulatory Networks During Herba Gelsemium elegans Intoxication

doi: 10.3389/fphar.2019.01217

Figure Lengend Snippet: circRNA-miRNA-mRNA co-expression network. The co-expression network was constructed by Cytoscape software based on conjoint analysis of circRNA and mRNA microarray results. Red nodes represent miRNAs, light-blue nodes represent mRNAs, and brown nodes represent circRNAs. The interconnected nodes represent direct relationships.

Article Snippet: The labeled mRNAs and circRNAs were separately hybridized onto the Whole Mouse Genome Oligo Microarray (4×44K, Agilent Technologies, Santa Clara, CA) and the Arraystar Mouse circRNA Array V2 (8×15K, Arraystar Inc., Rockville, MD).

Techniques: Expressing, Construct, Software, Microarray

Journal: Frontiers in Pharmacology

Article Title: Construction and Analysis of circRNA-miRNA-mRNA Molecular Regulatory Networks During Herba Gelsemium elegans Intoxication

doi: 10.3389/fphar.2019.01217

Figure Lengend Snippet: Enrichment analysis of (A) GO term and (B) pathway term for circRNA co-expression genes. The top 10 changed GO/pathway terms were drawn in enrichment bubble diagrams. Bubble scales represent the number of differently expressed genes. The depth of the bubble color represents the p-value.

Article Snippet: The labeled mRNAs and circRNAs were separately hybridized onto the Whole Mouse Genome Oligo Microarray (4×44K, Agilent Technologies, Santa Clara, CA) and the Arraystar Mouse circRNA Array V2 (8×15K, Arraystar Inc., Rockville, MD).

Techniques: Expressing

Journal: Frontiers in Pharmacology

Article Title: Construction and Analysis of circRNA-miRNA-mRNA Molecular Regulatory Networks During Herba Gelsemium elegans Intoxication

doi: 10.3389/fphar.2019.01217

Figure Lengend Snippet: The key function module of circRNA-miRNA-mRNA network. The network was drawn based on the biological function of circRNA co-expression genes. Nodes in red are miRNAs, nodes in light-blue are mRNAs, and nodes in brown are circRNAs. The interconnected nodes represent direct relationships.

Article Snippet: The labeled mRNAs and circRNAs were separately hybridized onto the Whole Mouse Genome Oligo Microarray (4×44K, Agilent Technologies, Santa Clara, CA) and the Arraystar Mouse circRNA Array V2 (8×15K, Arraystar Inc., Rockville, MD).

Techniques: Expressing